INFORMATIONS ABOUT PEPTIDES
WHAT ARE PEPTIDESFrom a chemical point of view, the peptide is an organic chemical compound formed by the merger of several amino acids. Individual amino acids bound (in a defined order of succession and calling the sequence) while they are connected by the so-called. an amide bond, in one branched chain – peptide. The pioneering approach of peptides in biochemistry and organic chemistry, this relationship is often called as a peptide bond. We denote peptides of relatively short amino acid sequences with 50 (generally more than 100) amino acids. The peptides of the protein (proteins) differ only in size (the number of amino acids included in the chain), the separation between the peptides and the proteins is not entirely clear and is determined by agreement, in which the considered line of the 100 amino acids. Naturally occurring peptides are composed mostly through the process of protein biosynthesis. The actual sequence information (sequence of amino acids) are encoded in DNA. Peptides in the body perform a number of important and irreplaceable functions. Peptide hormones and neuropeptides regulate most processes in the human body. • The peptides are involved in the recovery and growth of cells, the proper functioning of the immune system, body metabolism, regulation of sleep, hormone production, etc. • Peptides affect many physiological factors, such as growth and quality muscle mass, performance, endurance, overall fitness, burn body fat, regeneration of all tissues, organs, joints and tendons, wound healing and many others. • Peptides well affect the mental factors such as memory, mood, behavior and concentration. Promoting quality, deep sleep and a positive effect on overall mental and mental freshness, vitality and energy. • Peptides positive impact on a lot of factors that are essential for good health. Strengthen immunity, increase metabolism, lower cholesterol, promote and strengthen heart function, blood vessels, brain, liver, kidneys and other internal organs. Further increases bone density, stimulates red blood cell production, and total body cleanse (antioxidant). • Peptides capable of very effective protection against serious diseases and health problems such as cardiovascular disease, obesity, diabetes and metabolic disorders, cancer, wasting and weakness (muscular dystrophy), bone loss, mental disorders, negative effects of stress and depression (neuroprotective effects), skin diseases, various infections and inflammations, hormonal and sexual disorders, infertility and more. • Peptides can protect and even, for example, from skin cancer and also gives your skin a dark Otieno strong and lasting tan. • Peptides can eliminate and remove serious aesthetic defects and defects. At the same time significantly slows the aging process for their rejuvenating and regenerative effects. Among the aforementioned benefits include the efficient extermination or completely eliminate wrinkles, soften and rejuvenate the skin, strengthen hair growth, etc. Peptides have tremendous potential in the future to ensure ideal human physical and mental condition, regeneration, freshness, vitality, youthfulness, longevity, good health and overall very high quality of life because they have the unique power to cause positive changes in the necessary biochemical processes in the body. Unlike drugs or anabolic steroids, peptides act without their side and harmful effects. All changes in the body that are caused by their effects take place in its natural form. For the proper functioning of cells, tissues, organs, and ultimately, substantially the entire body should be within a sufficient amount of peptide needed. However, with age (after almost 20 years of age) begins to grow in the body lack peptides (their natural production decreases and becomes increasingly steadily, while decline in production of its own endogenous growth hormone). Lack of peptides and endogenous growth hormone significantly accelerates the deterioration of tissue, general health and functioning of the whole organism, and also starts the process of rapid aging of the organism. The fact that the researchers were able to successfully synthesize many peptides with many beneficial effects on the human body, causing real and absolute revolutionary break and outlined the possible future use of peptides not only in good health, beauty, high quality of life and the prevention and treatment of diseases, but also in the world sport.
Definition and side effects of GHRP Peptides
GHRP peptide:There are two categories of individual peptides of growth hormone. These two categories are called. GHRH (growth hormone releasing hormone) and GHRP (growth hormone releasing peptides). GHRH peptides increase the amount of growth hormone, which can be secreted at times that are natural for the body. GHRP peptides stimulate pulse that stimulates the pituitary gland to eliminate the growth hormone stored therein. Although our body continues to produce growth hormone (RH) or in old age, but it does not have in an amount such as in early age when we grow. During the day there are certain periods when the human body is ready to release the stored growth hormone. Interestingly, the most significant release of RH occurs when we sleep (this is why sleep is important for children). If you are interested in building muscle mass, then you know that sleep and food intake are two of the most important factors that you need to constantly observe. Use of peptides from the group as GHRP sekratagógov growth hormone (GHS) has been studied extensively. Scientists have found that the growth hormone releasing peptides increase the natural production of growth hormone (GH). • Hexapeptide GHRP-6 is a potent stimulator of the natural release of growth hormone. GHRP-6 is a specific activator of artificial newly discovered receptor called growth hormone receptor sektretagógu (GHS-R). Before long, it was discovered ghrelin, an endogenous ligand that binds to GHS-R. Synthetic ghrelin also have a compound as GHRP-6 is named “growth hormone secretagogues.” One side effect of the GHRP-6 was a significant increase in appetite, whereas stimulates the release of ghrelin, a peptide which is naturally excreted in the walls of the stomach and increases the appetite and gastric emptying. GHRP-6 stimulates the anterior pituitary, which increases the release of growth hormone. Increased amounts of growth hormone may increase the secretion of the hormone IGF-1 in the liver, which improves the body’s ability to burn fat and build muscle. Whereas GHRP-6 acts directly on a feedback loop that indicates the inhibition of growth hormone release, it can re-stimulate the production of growth hormone. • GHRP-2 (also known as KP 102) is a synthetic hexapeptide growth hormone releasing peptide (GHRP), which acts on the hypothalamus and the pituitary and thereby promoting growth hormone release with a slight stimulatory effect on prolactin, ACTH and cortisol. GHRP-2 is considered to be the true growth hormone secretagogue as stimulate the secretion of its own RH. GHRP-2 belongs to the second generation GHRPs immediately after GHRP-sixth Compared with the GHRP-6, GHRP-2 to be more effective in terms of stimulation of growth hormone, since maintaining a maximum increase of growth hormone levels for longer. It has been shown that GHRP-2 increases the levels of IGF-1 (insulin-like growth factor 1). • Ipamorelin hormone it is the pentapeptide (Aib-His-D-2-Nal-D-Phe-Lys-NH 2), a growth hormone secretagogue ghrelin mimetics, small molecule, developed by Novo Nordisk. Ipamorelin belongs to the latest generation of GHRP and largely releases growth hormone. As GHRP-6 and GHRP-2 and inhibits the somatostatin stimulate and increase the release of growth hormone (GH) from the anterior pituitary. Ipamorelin belongs to the third generation of GHRP-6, GHRP, and GHRP-2. Ipamorelínu properties resemble GHRP-2: no lipogenic properties of ghrelin, does not hungry. On the other hand ipamorelin similar to GHRP-6, because both GH release with very similar intensities without the side effects of GHRP-sixth GHRP-6 and GHRP-2 and causes the release of cortisol and increases levels of prolactin. Ipamorelin not only selectively releases RH, and at any dose. Ipamorelin has the same effect as hexarelin. Compared to other peptides is a much more stable form of ghrelin, and has a longer half-life (at least two hours. • Hexarelin acetate is a synthetic hexapeptide of a number of growth factors, which stimulate the release of growth hormone (GH) and does not interfere with the ability of the body to create its own GH. Structurally, the hexarelin (hexarelin acetate) similar to GHRP-6, but does not increase the appetite, as they can not significantly increase the levels of ghrelin, which is responsible for increased appetite and faster gastric emptying. Hexarelin is a synthetic growth hormone secretagogue consisting of six amino acids. It has a strong ability to release growth hormone in the human body. The increase of circulating GH caused Hexarelin increases the levels of insulin-like growth factor (IGF-1) in the liver. Hexarelin (hexarelin acetate) is a potent analog of GHRP-sixth The strength of the peptide should not be underestimated, because its use is associated with increased production of cortisol and prolactin. By far not close to the increased levels of the anabolic steroid, but a GHRP other has the strongest affinity to increased cortisol / prolactin. In most of the studies with the peptides of the GHRP was used as defined in saturation dose amount of 100 mcg or 1 mcg of active substance per 1 kg of weight. Thus, the dose of 100 mcg sufficiently saturated with receptors. Therefore, it is considered a loading dose of 100 mcg and adding another 300 or 400 mcg would have seen only a slightly higher effect. 500 mcg dose will be much more effective than 400 mcg, maybe even 300 mcg. Other problems include the desensitization and side effects of cortisol and prolactin. • GHRP-6 at saturation dose of 100 mcg in fact does not increase prolactin or cortisol but can be done at slightly higher doses. This increase is still in the normal range. • Effective GHRP-2 stimulate growth hormone release than GHRP-6, but at a higher dose, or saturation may slightly increase the levels of prolactin and cortisol. This increase is still in the normal range, although doses of 200-400 mcg could represent the end of the normal range. • Ipamorelin has approximately the same effect on the release of growth hormone such as GHRP-6, but not at higher doses (above 100 mcg) does not create a prolactin or cortisol. • Hexarelin in some cases the most effective. On average, the effect of stimulating the release of growth hormone equipotent (same), or slightly less than GHRP-2. However, it has the greatest potential to increase the levels of cortisol and prolactin. This increase will occur even when the saturation dose of 100 mcg. This growth will reach a higher level than the level defined as normal.
Recommended dosage:1. GHRP-2 and GHRP-6 is recommended to use at a dose of 100-200 mcg. At this higher dose than the saturation dose it is necessary to pay attention to desensitization and increased levels of prolactin and cortisol. 2. ipamorelin recommended to use at a dose of 100 to 2000 mcg (2 mg) without any problems. At high doses it does not produce prolactin and cortisol, and does not cause any problems with the pituitary gland. Desensitization does not occur even at higher doses. 3. hexarelin recommended to use at a dose of 100-150 mcg. Desensitization and increased levels of prolactin and cortisol occurs already saturating dose (100 mcg).
Glucocorticoid, cortisol and prolactin:Glucocorticosteroids or glucocorticoid steroid hormones are formed by the adrenal cortex. Their creation governed by the anterior pituitary ACTH production. The main figures are cortisol and corticosterone. cortisol is controlled corticotropin (ACTH), a hormone of the pituitary gland. Cortisol itself is formed in the middle layer adrenal cortex, the daily production is between 15 and 30 mg. It is transmitted by blood, protein-bound transkorntín. Its main function is to increase the overall readiness in situations of stress. Cortisol regulates the intermediate metabolism, lipid metabolism, protein and carbohydrate, increases the level of blood sugar (glucose) in the blood by gluconeogenesis, suppress the immune system, reduces inflammation and reduces symptoms of bone formation. Prolactin (luteomammotropný hormone, PRL) is a hormone produced by the pituitary type chromophilic cells (LTH – mammotropné cells). Induces secretion of prolactin and human milk, together with other hormones involved in the maintenance thereof, stimulate the proliferation of mammary cells, inhibition of hormone production by the ovaries at an elevated level, affects the fluid and electrolyte homeostasis, metabolism of glucose and the fluid, fat digestion in the body.
Conclusion:The use of peptides GHRP-2, GHRP-6 and Hexarelin at much higher doses than the saturation dose results in increased production of cortisol and prolactin. By far, this is not close at increased levels of anabolic steroid use. Since these peptides act directly on a feedback loop that indicates the inhibition of growth hormone release, re-stimulation of the natural growth hormone after the failure is possible.
INFORMATION ON THE SOLUBILITY OF PEPTIDES
Some peptide solution is slightly turbid (eg. HGH Fragment 176-191, Sermorelin). It is their normal and natural property determined by their structure, not about any flaw. If it happens that the peptides in solution immediately after dilution completely dissolved (float tiny suspended particles / fragments), first make sure that you have added to the tube at a sufficient amount of the solution (1-2 ml). In some cases, the contents within the ampoule is completely dissolved in the solution up to the next 24 hours. Nevertheless, you can also still completely insoluble content. It is safe, functional and is in no way contaminated. Peptide manufacturers indicate that the difference in efficiency did not change significantly due to the very small amount of remaining completely still undissolved peptide in solution.
Moisture, fillers and additives play an important role in the bulkiness of the lyophilized powder. Where the lyophilized powder contains no filler, it is less likely that the peptides will appear bulky. The more moisture in the freeze-drying process, the peptide is bulkier look and the easier it will dissolve. If the drying process is eliminated the majority of the residual moisture, the peptides are reduced and will appear to be a close or tight clusters of balls. This is the best condition for long-term storage. With proper storage temperature (-20 ° C) with no moisture or oxygen the remaining freeze-dried peptides stable up to a decade. Without built-in humidistat may be difficult to develop their structure or dissolve them.
Short chain peptides, with very little tertiary structure are not brittle (e.g. GHRP-2, GHRP-6, ipamorelin, ModGRF 1-29). ModGRF 1-29 peptide is produced with a modification 4, 2 of which it resistant during synthesis. As a result of these adjustments is not mentioned peptide very fragile. Peptide Sermorelin is more fragile and peptide CJC-1295 DAC + is fragile position in the lysine linker.
Each amino acid has unique properties that determine how it will behave in relation to water. The peptides can thus more or less attract or repel water. Some peptides have a tendency to retain more residual water in the freeze-drying process and act accordingly visually bulky. Peptides that visually appear to be less bulk are more likely to adhere to a glass vial. Reconstitution of their solution is much more demanding.
THE USE OF THE PEPTIDES FROM GHRP AND GHRH GROUPS
1. DOSAGE GHRP Peptides
In most of the studies with the peptides of the GHRP was used as defined in saturation dose amount of 100 mcg or 1 mcg of active substance per 1 kg of weight. Thus, the dose of 100 mcg sufficiently saturated with receptors.
– If this dose to a further 100 mcg, will be effective only 50% of the amount added.
– In case of a dose of a further 100 mcg, the active only about 25% of the amount added.
– Another 100 mcg moreover, could only slightly contribute to a stronger release of growth hormone.
Therefore, it is considered a loading dose of 100 mcg and adding another 300 or 400 mcg would have seen only a slightly higher effect. 500 mcg dose will be much more effective than 400 mcg, maybe even 300 mcg. Other problems include the desensitization and side effects of cortisol and prolactin.
• ipamorelin has approximately the same effect on the release of growth hormone such as GHRP-6, but not at higher doses (above 100 mcg) does not create a prolactin or cortisol.
• GHRP-6 at saturation dose of 100 mcg in fact does not increase prolactin or cortisol but can be done at slightly higher doses. This increase is still in the normal range.
• effective GHRP-2 stimulate growth hormone release than GHRP-6, but at a higher dose, or saturation may slightly increase the levels of prolactin and cortisol. This increase is still in the normal range, although doses of 200-400 mcg could represent the end of the normal range.
• Hexarelin in some cases the most effective. On average, the effect of stimulating the release of growth hormone equipotent (same), or slightly less than GHRP-2. However, it has the greatest potential to increase the levels of cortisol and prolactin. This increase will occur even when the saturation dose of 100 mcg. This growth will reach a higher level than the level defined as normal.
2. Peptides GHRP desensitization
(Definition desensitization: Receptor desensitisation)
• GHRP-6 and GHRP-2 may be used in the saturation dose three or four times a day without the risk of desensitization.
• Chronic daily use GHRP-6 and GHRP-2 at a dose of 100 mcg applied several times a day will not cause any problems with the pituitary gland or significant problems in terms of prolactin and cortisol, or desensitization.
• has been shown to cause desensitization Hexarelin. The long-term study pituitary renewed its sensitivity so as not to be loss of sensitivity at the saturation dose. However, the use of Hexarelin at 100 mcg three times a day is likely to lead to some 14 days to “down-regulation”. Where ever the desensitization occurred for either of these peptides GHRP lines, simply to stop taking several days, and this effect will disappear.
3. Releasing peptide hormone GHRH
Sermorelin (GRF 1-29) and GHRH 1-44 belong to a group GHRH peptides. They have a short half-life in plasma by a rapid cleavage between amino acid 2 and 3. It is not a cause for concern since GHRH hormone is secreted from the hypothalamus and travels only a short distance to the anterior pituitary, so in fact the enzymatic cleavage does not occur. The release from the hypothalamus and binding to somatotropin cells (cells of the pituitary gland) occurs rapidly. However, if the hormone is applied to the body by injection, the first circulating path until you find the pituitary and therefore has been degraded over 3 minutes. That is why the peptides to be in the above forms administered at high doses, to achieve the effect.
4. GHRH analogues Peptides
GHRH peptide analogues typically exchanged at the position 2 alanine for D-alanine, which makes the peptide resistant to rapid cleavage at this position. Thus, the analogs administered in lower doses will be effective. Tetrasubstituted GRF 1-29, sometimes also called CJC-1295 without DAC or Modified GRF 1-29 (Modified GRF 1-29) has a different amino acid modifications. It is a Glutamine (Gln or Q) in the 8-position, alanine (Ala or A) at position 15 and the leucine (Leu or L) in the 27-position. Alanine at the 8 position to increase the bioavailability. Two other amino acid substitutions are made to improve the production process (i.e., a processing stability).
In the in vivo in humans is GHRH analogue Modified GRF 1-29 (CJC-1295 without DAC) highly effective peptide-lived probably 30 minutes or more. This is long enough to be fully effective. Therefore, saturation dose is defined at 100 mcg or 1 mcg of active substance per 1 kg of weight.
5. The use of unopposed GHRH PEPTIDE
The problem with the use of GHRH peptides (and their strongest analog) is as follows. Said peptides are highly efficient only when the low level of somatostatin (growth hormone inhibiting hormone). If the GHRH peptide apply at the time when the lowest level (or when there is no pulse natural growth hormone), to achieve the release only a small amount of growth hormone. However, if you are lucky and submit it during rising levels of growth hormone (somatostatin at this point is not active), increases the release of growth hormone.
6. The combination of GHRP and GHRH Peptides
This solution is simple and highly effective. It is recommended to use a peptide analogue of GHRH with GHRP peptide. The peptides GHRP the form of a pulse of growth hormone. They do so through several mechanisms. One mechanism is the reduction of the release of somatostatin from the hypothalamus. Another mechanism is to reduce the impact of somatostatin on pituitary. Another mechanism of increasing the release of hormones from the brain of GHRH. GHRP peptides are active at the same pituitary cells (cells somatotropin) from the group of peptides such as GHRH, but use a different mechanism to increase of cAMP, which in turn causes the release of growth hormone from the somatotropic cells. GHRH peptides can also reciprocally potentiate the effects GHRP peptides from the group. The result is a synergistic release of growth hormone. Growth hormone is not additive, but synergistic.
One common SYNERGY GHRP and GHRH Peptides:
– GHRH peptide causes release of RH rated by 2 points
– GHRP peptide causes release of RH rated 5 points
– Total due to release RH rated 7 points
(Sum of points GHRP and GHRH 5 + 2) but, for example, up to 14 points
7. DOSAGE COMBINATION GHRP and GHRH Peptides
• The most suitable dosage regimen for the peptide combination of the above groups before bedtime dose administration (to aid night pulse), and another 2 to 3 applications of doses throughout the day (e.g. in morning, before training, during the day).
• In the event that your goal is to use the rejuvenating effects, respectively. anti-aging (anti-aging efect), peptides sufficient dose once a day at bedtime. For a man aged 40 years and over it is fully sufficient to restore the levels of growth hormone level of a young person.
• If appropriate, use in body, and the reduction of fat in the treatment of wounds are effective multiple doses administered several times a day.
• GHRH analogues of peptides (eg. Sermorelin and Modified GRF 1-29) is recommended to take at a dose of 100-200 mcg. Higher doses than those mentioned do not cause any problem.
• GHRP-2 and GHRP-6 can be taken at a dose of 100 – 200 mcg without any problems. Again, it is necessary to pay attention to desensitization and increased levels of prolactin and cortisol at higher doses.
• ipamorelin can be taken at a dose of 100 – 4000 mcg (4 mg) without any problems. At high doses it does not produce prolactin and cortisol, and does not cause any problems with the pituitary gland. Desensitization does not occur even at higher doses.
• hexarelin can be taken at a dose of 100-150 mcg. Desensitization and a significant increase in levels of prolactin and cortisol occurs already saturating dose (100 mcg).
• The effective dosage when co-administered in the amount of 100-200 mcg GHRP peptides together with a number from 100 to 200 mcg for GHRH analogues of peptides. These quantities is recommended doses several times a day to achieve a high effect.
• When dosing multiple times a day is necessary to maintain the spacing between doses in the range of 3 hours.
– All the peptides to be administered without food, or just after consumption of protein alone.
– Fats and carbohydrates attenuate the release of growth hormone.
– After use of the peptides need to withstand fasting for at least 20 minutes (no more than 30, but not less than 15 minutes), growth hormone as the pulse reaches its peak.
INFORMATIONS OF STORAGE PEPTIDES
1. Storage lyophilized peptide (before dilution with sodium)
The peptides are supplied as a lyophilized powder. Short-term temperature fluctuations do not affect the quality and efficacy of the product during transport. At room temperature, the peptides are stable in lyophilized form for several weeks. Lyophilized peptides recommended to store in a freezer at -20 ° C. The vast majority of lyophilisates can be stable at this temperature for a few years. It is advisable to avoid frequent repetition of the process of freezing and thawing peptides.
2. Storage The reconstituted peptide (after dilution with a solution)
The reconstitution of the lyophilized powder the process of dilution with a solvent. The recommended solvent is a physiological saline solution (0.9% NaCl). Before reconstitution of lyophilized peptides leave tempered to room temperature. After reconstitution, the life of a peptide restricted. Reconstituted peptides recommended to store in a refrigerator at 2-8 ° C. The vast majority of the peptides in solution may be stable at this temperature for 2 weeks. Storage at -20 ° C may be the peptides in the solution stable for 3 months. It is advisable to avoid frequent repetition of the process of freezing and thawing peptides.
Where Can I buy peptides?
We offer and sell high quality of research peptides manufactured by European based company providing chemicals for R&D purposes in accordance with our EU member country and European Chemical Agency legislation. Our company supply research community with world-class products through the online store and guarantee fast international shipping with amazing on-time success delivery rate. The purity and integrity of our EU made peptides and proteins is extremely important to us and to our customers who will be able to achieve top of the line laboratory research at extreme low prices. Through the latest SSL security technology, and a user-friendly website design, we have created an environment where customers can place orders simply, quickly and 100% secure. Experienced professional customer support team is prepared to help in a case of need. Research chemicals are produced in a state of the art facility by one of the world leaders in peptide synthesis, gene synthesis, protein and antibody production under strict European Union manufacturing regulations. Our products undergo a strict quality control assessment that is unmatched in the industry. All peptides and proteins are stored in controlled -20°C conditions to avoid any quality degradation. High level of purification refine >98% purity depending on complexity. We also offer a diverse spectrum of modifications. HPLC analysis and Mass Spectrometry analysis performed on every batch proves declared quality. We are proud to be a trusted resource for the products we provide to the scientific community or to each customer familiar with our trading terms.